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Image Search Results
Journal: Mucosal immunology
Article Title: Interleukin (IL)-21 promotes intestinal IgA response to microbiota
doi: 10.1038/mi.2014.134
Figure Lengend Snippet: ( A ) Fecal pellets were collected from age-matched wild-type, IL-21 −/− , and IL-21R −/− B6 mice. IgA levels were quantified through ELISA and normalized to total protein. N = 2 mice per group, data is reflective of 2 independent experiments. *p<0.05 compared to wild-type mice. ( B ) Naïve IgD + B cells were activated with anti-µ (5µg/ml) and anti-CD40 (5µg/ml) in the presence of TGFβ1 (5ng/ml), IL-17 (50ng/ml), IL-21 (20ng/ml), or retinoic acid (1µM), or a combination of TGFβ1, IL-17, IL-21, and/or retinoic acid. On day 5, IgA was measured from the supernatant by ELISA. Data is reflective of 6 independent experiments. *p<0.05, ***p<0.001 compared between samples. + p<0.05, ++ p<0.01 compared to untreated cells.
Article Snippet: The following antibodies were used for flow cytometry: PE-IL-17A (TC11-18H10.1), FITC-B220 (RA3-6B2), PE-α 4 β 7 (DATK32), FITC-CD4 (RM4-5), PE/Cy7-CD38 (90), APC-CD138 (281-2), and PE/Cy7-PD-1 (29F.1A12) were purchased from Biolegend; APC-RORγt (B2D), APC-GL-7, and eFluor450-Streptavidin were from eBioscience; Brilliant Violet 421-CD95 (Jo2) and biotinylated CXCR5 (2G8) were from BD Biosciences;
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Mucosal immunology
Article Title: Interleukin (IL)-21 promotes intestinal IgA response to microbiota
doi: 10.1038/mi.2014.134
Figure Lengend Snippet: 10 6 CBir1 Th17 cells were i.v. transferred into TCR®x™ −/− mice. ( A ) On day 44 post-Th17 cell transfer, expression of surface IgA on splenic, intestinal lamina propria, or Peyer’s patch B cells of CBir1 Th17 cell recipients, Th17 cell recipients receiving neutralizing IL-21r (Th17+αIL-21r), or control TCRβxδ −/− mice was determined by flow cytometry. FACS plots are representative of 4 mice per group. ( B ) IgA + plasma cells and mature B cells in the spleens and intestines. Bar charts reflect mean +/− SEM. Data reflects 3 independent experiments. *p<0.05, **p<0.01, ***p<0.001 between compared samples. LPL = intestinal lamina propria lymphocytes; PP = Peyer’s patch; PC = plasma cell
Article Snippet: The following antibodies were used for flow cytometry: PE-IL-17A (TC11-18H10.1), FITC-B220 (RA3-6B2), PE-α 4 β 7 (DATK32), FITC-CD4 (RM4-5), PE/Cy7-CD38 (90), APC-CD138 (281-2), and PE/Cy7-PD-1 (29F.1A12) were purchased from Biolegend; APC-RORγt (B2D), APC-GL-7, and eFluor450-Streptavidin were from eBioscience; Brilliant Violet 421-CD95 (Jo2) and biotinylated CXCR5 (2G8) were from BD Biosciences;
Techniques: Expressing, Control, Flow Cytometry, Clinical Proteomics
Journal: Mucosal immunology
Article Title: Interleukin (IL)-21 promotes intestinal IgA response to microbiota
doi: 10.1038/mi.2014.134
Figure Lengend Snippet: ( A ) On day 44 post-Th17 cell transfer, IgG and IgA in the serum was measured by ELISA. *p<0.05, **p<0.01, ***p<0.001 ( B ) Fecal pellets were collected from Th17 cell recipients during the course of the experiment. IgA levels were quantified through ELISA and normalized to total protein. Changes in expression over time are expressed as a fold change from individuals pre-transfer. N = 4 mice per group, from 3 independent experiments. *p<0.05 as compared to anti-IL-21r group. ( C ) Intracellular IgA production was measured from total bone marrow cells of CBir1 Th17 cell recipients, Th17 cell recipients receiving neutralizing IL-21r (Th17+αIL-21r), or control TCRβxδ −/− mice by flow cytometry. FACS plots are representative of 4 mice per group.
Article Snippet: The following antibodies were used for flow cytometry: PE-IL-17A (TC11-18H10.1), FITC-B220 (RA3-6B2), PE-α 4 β 7 (DATK32), FITC-CD4 (RM4-5), PE/Cy7-CD38 (90), APC-CD138 (281-2), and PE/Cy7-PD-1 (29F.1A12) were purchased from Biolegend; APC-RORγt (B2D), APC-GL-7, and eFluor450-Streptavidin were from eBioscience; Brilliant Violet 421-CD95 (Jo2) and biotinylated CXCR5 (2G8) were from BD Biosciences;
Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Control, Flow Cytometry
Journal: Mucosal immunology
Article Title: Interleukin (IL)-21 promotes intestinal IgA response to microbiota
doi: 10.1038/mi.2014.134
Figure Lengend Snippet: 10 6 CBir1 Th17 cells were i.v. transferred into TCR®x™ −/− mice. ( A ) On day 44 post-Th17 cell transfer, expression of germinal center markers GL-7 and CD95 on splenic, mesenteric lymph node, intestinal lamina propria, or Peyer’s patch B220 + cells of CBir1 Th17 cell recipients, Th17 cell recipients receiving neutralizing IL-21r (Th17+αIL-21r), or control TCRβxδ −/− mice was determined by flow cytometry. FACS plots are representative of 4 mice per group. ( B ) IF image of Peyer’s patch from control TCRβxδ −/− mice or CBir1 Th17 cell recipients with 20× objective. Germinal center formation was analyzed by staining for GL-7-AlexaFluor 594 and B220-FITC. ( C ) RNA was collected from ileal tissue on day 44 post-Th17 cell transfer from CBir1 Th17 cell recipients or control TCRβxδ −/− mice and Aicda mRNA was analyzed by RT-PCR. Data is reflective of 4 mice per group, from 3 independent experiments.
Article Snippet: The following antibodies were used for flow cytometry: PE-IL-17A (TC11-18H10.1), FITC-B220 (RA3-6B2), PE-α 4 β 7 (DATK32), FITC-CD4 (RM4-5), PE/Cy7-CD38 (90), APC-CD138 (281-2), and PE/Cy7-PD-1 (29F.1A12) were purchased from Biolegend; APC-RORγt (B2D), APC-GL-7, and eFluor450-Streptavidin were from eBioscience; Brilliant Violet 421-CD95 (Jo2) and biotinylated CXCR5 (2G8) were from BD Biosciences;
Techniques: Expressing, Control, Flow Cytometry, Staining, Reverse Transcription Polymerase Chain Reaction